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KMID : 0545120010110010061
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 1 p.61 ~ p.64
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Enhanced Transformation Efficiency of an Anticoagulant Hirudin Gene into Saccharomyces cerevisiae by a Double δ-Sequence
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Kim, Myoung Dong
Yoo, Young Je/Rhee, Sang Ki/Seo, Jin Ho
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Abstract
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Delta-integration vectors were constructed for the purpose of achieving homologous integration of the hirudin expression cassette into the chromosome of Saccharomyces cerevisiae. A double δ system truncated with the unnecessary bacterial genes, and consequently having a reduced insert size for integration, showed a four-fold increase in transformation efficiency at given DNA concentrations, and as a result, the constructed recombinant yeast strain had a 1.3-fold enhancement in hirudin expression level compared with a single δ system.
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